The accident happened around 10:30 a.m. Monday about a mile west of Clarks in Merrick County.
Heather Stroud was headed east on Highway 30 when her vehicle was struck from behind by a semi driven by 47-year-old James Horton of Roseland. The impact knocked Stroud's vehicle into the westbound lane where it was struck by an oncoming car.
Stroud was killed and the driver of the oncoming car was injured. Horton was not injured.
The accident is still under investigation.